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World BioHazTec has been a leader in biosafety and biosecurity since its inception in 1995. Over the years, we have successfully completed numerous groundbreaking projects and received prestigious awards, showcasing our dedication to excellence and innovation.

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HVAC air ducts

Selecting BSL-3 Supply Air Duct Material and Decontamination

When considering the material for BSL-3 supply air ducts, the primary objective of biosafety design is to ensure a continuous duct path from the room’s supply diffusers and exhaust outlets (including biological safety cabinets [BSCs]) to ideally an airtight valve in the supply and exhaust ductwork, respectively. This sealed duct section not only maintains the concentration of decontamination gas or vapor required for efficacy but also prevents any leakage of the decontamination agent into surrounding rooms. We advocate for two options while advising against two other alternatives. Selection should be based upon a risk assessment of your facility’s ductwork design. Option 1: Welded Stainless Steel Ductwork In the context of BSL-3 facilities, employing welded stainless steel ductwork for both exhaust and supply systems is recommended. This ductwork extends from the BSL-3 laboratory to the airtight isolation damper of the HEPA caisson inlet and the low leakage shut-off control air valve or isolation valve. These supply and exhaust ductwork sections are referred to as “decon duct sections” to distinguish them from ductwork sections where the decon agent is not held for decontamination efficacy. During the decontamination process, the closure of only these two airtight valves is necessary, aligning with current design practices. This approach provides the necessary airtight duct sections for the supply and exhaust decon duct sections. In terms of operational ease, the preparation for decontamination is streamlined through a dedicated button on the Building Automation System (BAS) interface. However, even with the implementation of welded stainless steel ductwork, it remains imperative to conduct integrity testing of the duct decon sections during the commissioning phase. This precaution is essential as the risk of potential welding pin holes in the ductwork hinges upon the proficiency of the welder involved. Option 2: Galvanized Ductwork With Sealed Joints An alternative to Option 1 is round galvanized steel ductwork with press fit joints, where all duct joints are sealed with duct mastic to ensure airtightness. Rectangular or square ductwork can be flanged with neoprene gaskets. Like Option 1, readiness for decontamination requires closing the two airtight valves. It’s advisable to conduct an integrity testing during commissioning. Additionally, this design entails an annual visual examination of duct connections to detect any breaks or deterioration in the mastic at ductwork joints. The risk of ductwork mastic failure hinges on the meticulousness of the mastic application process. Option 3: Supply Spiral Galvanized and Welded Stainless Steel Flexible Ductwork (Not Recommended) We discourage the use of galvanized and welded seam stainless steel spiral flexible ductwork for the decontamination section. Welding may not be feasible due to the gauge of the metal. Option 4: Non-Metal Flexible (Not Recommended) This approach is discouraged. While some engineers have devised their own modifications, replicating the custom duct seal raises concerns particularly when dealing with a carcinogenic decontamination agent. Some material options offer unique benefits and considerations, ensuring the effectiveness and safety of BSL-3 laboratory decontamination operations. Option 3 and Option 4 should be avoided. Contact World BioHazTec to schedule a free 30-minute consultation or send us an email. You are a conversation away from starting down a successful pathway to meet BSL-3 facility compliance.

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scientist using a pipette in a BSL-3 facility

The 5 Strategic Advantages of Certifying Your BSL-3 Facility

Biosafety Level 3 (BSL-3) incidents are rare, yet their repercussions are monumental. Operating at BSL-3 demands meticulous protocols and unwavering risk mitigation. While rare, any lapses can have far-reaching and potentially devastating consequences. In this article, we explore the significance of BSL-3 certification. Even though it may not be mandatory in your country, it proves to be a vital strategic move. 1. Professional Accountability In a Biosafety Level 3 (BSL-3) facility, professional accountability emerges as the first compelling intention. Activities in a BSL-3 facility include the propagation of high-risk pathogens and long-term storage. This entails a profound responsibility. Researchers operating within BSL-3 facilities are accountable for ensuring not only their safety but also the safety of their colleagues, the community, and the environment. Certification acts as a tangible manifestation of this commitment, demonstrating to stakeholders and regulatory bodies that the organization embraces the gravity of their work. Beyond biosafety, BSL-3 certification also underscores the importance of stringent biosecurity measures, ensuring the safeguarding of these pathogens against unauthorized access and potential misuse. By obtaining BSL-3 certification, operators signal their dedication to maintaining the highest standards of both biosafety and biosecurity, fostering a culture of responsibility and accountability within the facility and beyond. 2. Public Health Preparedness Biosafety Level 3 (BSL-3) facilities play an indispensable role in public health preparedness. When a pandemic strikes, BSL-3 facilities stand at the forefront of the fight, equipped with the expertise and infrastructure necessary to handle and analyze high-risk pathogens. These facilities serve as vital hubs for research, diagnostics, and the development of therapeutic interventions. BSL-3 certification, in this context, becomes a strategic asset, signifying a commitment to being at the forefront of global health emergencies. It positions the facility to swiftly and effectively respond to emerging threats, contributing significantly to public health resilience and preparedness on a global scale. When the real test of a pandemic arrives, a certified BSL-3 laboratory signifies a prepared laboratory, with competent professionals adeptly executing their roles, ensuring a prompt and effective response to safeguard public health. 3. Creating a Safe and Secure Culture In a certified BSL-3 facility, a dedicated commitment to establishing a culture of biosafety and biosecurity is evident. This certification stands as a tangible testament to the management’s unwavering dedication to continuously enhancing biosafety and biosecurity measures. It mirrors a proactive approach towards nurturing a workplace culture that places the well-being of its staff and the integrity of its operations at the forefront. The certification process mandates rigorous adherence to standards, promoting continuous training and evaluation. This level of commitment resonates with the laboratory personnel, instilling confidence that their management is wholeheartedly invested in cultivating an environment where biosafety and biosecurity take precedence. 4. Safety Equals Quality A certified BSL-3 facility not only prioritizes safety and security but also indirectly contributes to the overall quality of results. Taking reference from the World Health Organization’s Handbook on Laboratory Quality Management System, laboratory safety is a cornerstone for establishing robust laboratory practices. By fostering a culture of strict adherence to biosafety protocols, certified BSL-3 facilities systematically integrate quality management principles into their daily operations. This meticulous approach not only safeguards the well-being of personnel but also ensures the integrity of laboratory processes. As safety practices become ingrained in the laboratory’s ethos following international quality management principles, the probability of errors and contamination diminishes, leading to consistently high-quality research outcomes. In essence, the commitment to safety in a certified BSL-3 facility reinforces the crucial connection between safety practices and the reliability of scientific results. 5. International Collaboration Opportunities A certified BSL-3 facility, known for its adherence to rigorous safety and security standards, builds credibility and trust within the scientific community. Collaborators and funding agencies, especially those from different countries, are more likely to engage with a facility that is recognized for its commitment to maintaining high-quality, safe, and secure research environments. BSL-3 facilities that demonstrate adherence to internationally recognized certification standards also make themselves attractive partners for collaborations assuring that research will be conducted in a secure environment, minimizing the likelihood of accidents or incidents. Conclusion In the pursuit of scientific excellence, BSL-3 certification emerges not merely as a commitment to safety but as a strategic gateway to collaborative possibilities. A certified BSL-3 facility, fortified by rigorous safety and security standards, amplifies credibility within the scientific community and encourages collaboration on a global scale. Let World BioHazTec be your strategic partner in preparing your facility to meet our certification requirements, widely acknowledged as the gold standard in BSL-3 certification. Contact World BioHazTec (WBHT) to schedule a free 30-minute consultation or send us an email. You are a conversation away from starting down a successful pathway to meet BSL-3 facility compliance.

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female scientist working with a petri dish

Five Design Considerations for Animal Laboratories

At the beginning of the design process, there are many decisions that must be made regarding equipment, sinks, showers, biological safety cabinets (BSCs), and other primary barrier equipment, the configuration of the HVAC system, and contingencies based upon the facility design elements required by the agents that will be worked with or studied. Sustainability, maintainability, and energy usage must also be factored into the design. Organizational preferred operations must be included, and SOPs often dictate final designs. Spatial relationships need to be evaluated to determine flow and function. When animals are part of the laboratory work, additional design features are needed which add complexity to the design. 1.  Design Standards, Guidelines, and Regulations for Animal Laboratories Paramount to safety is the understanding of the application and intent of biosafety and biocontainment guidelines. Biosafety design is based upon risk assessment. The design team should be accustomed to working with the Biosafety Officer, users, and stakeholders in formulating and documenting risk assessments when needed to support design decision-making. Determining your research goals or planned laboratory work, grant design requirements, and regulatory compliance will drive the design criteria. When designing an animal facility such as an ABSL-2 or ABSL-3 laboratory, beyond the compulsory local national and international guidance documents, you may want to also consider the following guidelines and standards: NIH Design Requirements Manual for Biocontainment Laboratories; U.S. Department of Agriculture (USDA) Research Service Guidelines, (as applicable); Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC); National Research Council Guide for the Care and Use of Laboratory Animals; National Research Council Occupational Health and Safety in the Care and Use of Research Animals; and National Research Council Occupational Health and Safety in the Care and Use of Nonhuman Primates. 2.  Odor  The management of odor from animal facilities, autoclaving, waste treatment, and tissue digesters is unique to containment facilities. Elevator shafts and loading docks serve as pathways for the distribution of odors. Using positive pressured elevator vestibules, adjoining positive pressured corridors, and local ventilation can contain odors. Dedicating an elevator for vertical movement of animals from receiving to the animal suite will also control odors. Adding a pneumatic waste removal system can contain waste removal odors from the animal suite to the building loading dock area. These engineering controls allow for the placement of vivariums anywhere vertically in a research tower instead of the traditional basement location. 3.  Noise Subjecting animals to noise and vibration can have significant impact on animal reproduction and sensory development, behavior, and can expose animals to injury startle responses. The location of rotating machinery in animal facilities can stress animals, so cage washing areas and mechanical spaces with fans and pumps need to be distanced from animal holding areas. Another source of noise is the ventilation system with elevated room level supply and exhaust noise. In addition to negatively affecting animals, this can also affect workers’ communication and awareness to their surroundings. 4.  Space Requirements Caging design, rack sizes and types, and animal model are key factors in analyzing space requirements. Identifying the maximum number of types of animals to be housed according to biosafety level can maximize space utilization for the present and the future. This information bears heavily on the design approach to room space allocation, cage washing equipment, whether disposable caging is more economical, cage changing stations or BSCs, and watering system versus bottle caging. 5.  Emergency Signaling Systems Placement of emergency signaling systems (e.g., fire alarm, HVAC failure alarm, room pressurization alarm, security alarm) is essential to alert personnel to act. Signaling systems must be accessible for lab personnel, biosafety officers, and emergency response personnel. Ever conscious of animals, the alarms cannot be strobing in holding facilities so as not to stress animals. Animal Laboratory Design Recommendations In analyzing the final design, emphasis needs to be placed on the details. Constant referral to the research program requirements, containment guidelines, lessons learned, project construction and operating budgets, and the completeness of the design documents are essential to a successful design that meets the users’ and the stakeholders’ needs and provides sustainability. Efforts spent in design by an integrated team of users, professionals, and stakeholders will culminate in a safe, sustainable, efficient, and secure research facility. Are you planning to build or renovate a BSL-3/ABSL-3 laboratory? No matter what project phase you are in, contact World BioHazTec (WBHT) to schedule a free 30-minute consultation or send us an email. We can prepare a feasibility study, develop conceptual designs with cost estimates, perform a site assessment, peer review design documents, commission/certify your animal facility, and train staff. You are a conversation away from starting down a successful pathway to meet containment compliance and sustainability.

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World BioHazTec is an Accredited Provider (AP) of the International Association for Continuing Education and Training (IACET). As an IACET Accredited Provider, World BioHazTec offers IACET CEUs for its learning events that comply with the ANSI/IACET Continuing Education and Training Information.

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